Mosloflavone is a flavonoid isolated from Scutellaria baicalensis Georgi with anti-EV71 activity.During the early stages of virus infection, mosloflavone inhibits the replication and protein expression of the VP2 virus and prevents the synthesis of the viral VP2 capsid protein. A promising biocide, mosloflavone prevents P. aeruginosa from being virulent and from forming biofilms.

Physicochemical Properties

Molecular Formula	C17H14O5
Molecular Weight	298.2901
Exact Mass	298.084
CAS #	740-33-0
PubChem CID	471722
Appearance	Light yellow to yellow solid powder
Density	1.3±0.1 g/cm3
Boiling Point	508.5±50.0 °C at 760 mmHg
Melting Point	163-164ºC (chloroform , acetone )
Flash Point	190.5±23.6 °C
Vapour Pressure	0.0±1.4 mmHg at 25°C
Index of Refraction	1.622
LogP	2.74
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	22
Complexity	440
Defined Atom Stereocenter Count	0
InChi Key	SIVAITYPYQQYAP-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H14O5/c1-20-14-9-13-15(16(19)17(14)21-2)11(18)8-12(22-13)10-6-4-3-5-7-10/h3-9,19H,1-2H3
Chemical Name	5-hydroxy-6,7-dimethoxy-2-phenylchromen-4-one
Synonyms	5-Hydroxy-6,7-dimethoxylflavone;F4DL1FN60Q;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: EV71; bacterial
ln Vitro	Mosloflavone inhibits TNF-α, IL-1β, and iNOS levels in the supernatant of mouse macrophage cell line J774A in a dose-dependent manner. It can also be used as a starting point to find lead structures for the treatment of inflammatory and immunomodulatory diseases[2]. Mosloflavone exhibits promising anti-inflammatory activity via inhibition of TNF-α and IL-1β with IC50 values of 16.4 uM and 6.4 uM, respectively.
References	[1]. Inhibitory Effects of Norwogonin, Oroxylin A, and Mosloflavone on Enterovirus 71.Biomol Ther (Seoul). 2016 Sep 1;24(5):552-8. [2]. Mosloflavone attenuates the quorum sensing controlled virulence phenotypes and biofilm formation in Pseudomonas aeruginosa PAO1: In vitro, in vivo and in silico approach.Microb Pathog. 2019 Jun;131:128-134. [3]. Anti-inflammatory and immunomodulatory flavones from Actinocarya tibetica Benth.Nat Prod Res. 2013;27(23):2227-30.
Additional Infomation	Mosloflavone is a member of flavonoids and an ether. Mosloflavone has been reported in Desmos dumosus, Mosla soochowensis, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~167.62 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (8.38 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.3524 mL	16.7622 mL	33.5244 mL
	5 mM	0.6705 mL	3.3524 mL	6.7049 mL
	10 mM	0.3352 mL	1.6762 mL	3.3524 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.