5,7-Dimethoxyluteolin, a 5,7-dimethylluteolin analogue, is a dopamine transporter (DAT) activator with EC50 of 3.417 μM.

Physicochemical Properties

Molecular Formula	C17H14O6
Molecular Weight	314.29
Exact Mass	314.079
CAS #	90363-40-9
PubChem CID	13964548
Appearance	White to light yellow solid powder
LogP	2.888
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	3
Heavy Atom Count	23
Complexity	476
Defined Atom Stereocenter Count	0
InChi Key	NKGJZNRUAGQIRY-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H14O6/c1-21-10-6-15(22-2)17-13(20)8-14(23-16(17)7-10)9-3-4-11(18)12(19)5-9/h3-8,18-19H,1-2H3
Chemical Name	2-(3,4-dihydroxyphenyl)-5,7-dimethoxychromen-4-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	EC50: 3.417 μM (Dopamine transporter)[1]
References	[1]. Discovery and synthesis of novel luteolin derivatives as DAT agonists. Bioorg Med Chem. 2010 Nov 15;18(22):7842-8.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (318.18 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.1818 mL	15.9089 mL	31.8177 mL
	5 mM	0.6364 mL	3.1818 mL	6.3635 mL
	10 mM	0.3182 mL	1.5909 mL	3.1818 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.