5,7-Dihydroxycoumarin is a coumarin extracted from the inflorescences of Macaranga triloba and has anti-bacterial effect.

Physicochemical Properties

Molecular Formula	C9H6O4
Molecular Weight	178.14
Exact Mass	178.026
CAS #	2732-18-5
PubChem CID	5324654
Appearance	Off-white to yellow solid powder
Density	1.6±0.1 g/cm3
Boiling Point	506.4±19.0 °C at 760 mmHg
Flash Point	216.9±15.0 °C
Vapour Pressure	0.0±1.4 mmHg at 25°C
Index of Refraction	1.689
LogP	0.86
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	0
Heavy Atom Count	13
Complexity	248
Defined Atom Stereocenter Count	0
InChi Key	KIQQFVJHWNCGAU-UHFFFAOYSA-N
InChi Code	InChI=1S/C9H6O4/c10-5-3-7(11)6-1-2-9(12)13-8(6)4-5/h1-4,10-11H
Chemical Name	5,7-dihydroxychromen-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Flavonoids with antiplasmodial and cytotoxic activities of Macaranga triloba. Fitoterapia. 2012 Jul;83(5):968-72. [2]. Synthesis and evaluation of antibacterial activities of 5,7-dihydroxycoumarin derivatives. Arch Pharm (Weinheim). 2011 Jun;344(6):386-93.
Additional Infomation	5,7-Dihydroxycoumarin has been reported in Haplophyllum dauricum, Fagopyrum megacarpum, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO : 25 mg/mL (140.34 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (14.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (14.03 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.6136 mL	28.0678 mL	56.1356 mL
	5 mM	1.1227 mL	5.6136 mL	11.2271 mL
	10 mM	0.5614 mL	2.8068 mL	5.6136 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.