4557W is a potent and reversible inhibitor of EGFR(Epidermal growth factor receptor) and c-ErbB2 (IC50s = 20 and 79 nM, respectively).

Physicochemical Properties

Molecular Formula	C23H21N3O3
Molecular Weight	387.439
Exact Mass	387.158
CAS #	179248-61-4
PubChem CID	9843206
Appearance	Off-white to gray solid powder
LogP	5.042
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	7
Heavy Atom Count	29
Complexity	480
Defined Atom Stereocenter Count	0
InChi Key	DNOKYISWMVFYFA-UHFFFAOYSA-N
InChi Code	InChI=1S/C23H21N3O3/c1-27-21-12-19-20(13-22(21)28-2)24-15-25-23(19)26-17-8-10-18(11-9-17)29-14-16-6-4-3-5-7-16/h3-13,15H,14H2,1-2H3,(H,24,25,26)
Chemical Name	N-(4-(benzyloxy)phenyl)-6,7-dimethoxyquinazolin-4-amine
Synonyms	4557 W; 4557W; 4557-W; EGFR/ErbB 2 Inhibitor; EGFR/ErbB-2 Inhibitor
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	EGFR/ErbB-2/ErbB-4 inhibitor-2 (compound 5) also suppresses c-Src, VEGFR2, and c-Fms, with IC50 values of 0.30, 2.2, and 14.0 μM, in that order [1].
References	[1]. Comparison of the biochemical and kinetic properties of the type 1 receptor tyrosine kinase intracellular domains. Demonstration of differential sensitivity to kinase inhibitors. J Biol Chem. 2002 Jan 11;277(2):1576-85.

Solubility Data

Solubility (In Vitro)

DMSO : ~33.33 mg/mL (~86.03 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.45 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.5810 mL	12.9052 mL	25.8104 mL
	5 mM	0.5162 mL	2.5810 mL	5.1621 mL
	10 mM	0.2581 mL	1.2905 mL	2.5810 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.