4-Nitrophenyl butyrate consists of butyric acid chains esterified with 4-nitrophenol groups, hence its yellow color. This compound is extensively used as a substrate in enzyme assays to measure esterase and lipase activity. When these enzymes cleave the ester bond, the nitrophenol group is released and the color changes from yellow to orange. Therefore, the rate of color change may be utilized to determine enzyme activity. In addition, 4-Nitrophenyl butyrate could be utilized as an organic synthesis reagent and dye intermediate.

Physicochemical Properties

Molecular Formula	C10H11NO4
Molecular Weight	209.20
Exact Mass	209.068
CAS #	2635-84-9
PubChem CID	75834
Appearance	Colorless to light yellow liquid（Density：1.19 g/cm3）
Density	1.2±0.1 g/cm3
Boiling Point	323.2±25.0 °C at 760 mmHg
Flash Point	144.8±25.2 °C
Vapour Pressure	0.0±0.7 mmHg at 25°C
Index of Refraction	1.534
LogP	2.55
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	4
Heavy Atom Count	15
Complexity	228
Defined Atom Stereocenter Count	0
SMILES	O(C1C([H])=C([H])C(=C([H])C=1[H])[N+](=O)[O-])C(C([H])([H])C([H])([H])C([H])([H])[H])=O
InChi Key	DVDUMIQZEUTAGK-UHFFFAOYSA-N
InChi Code	InChI=1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3
Chemical Name	(4-nitrophenyl) butanoate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	4-Nitrophenyl butyrate is a biochemical reagent that can be utilized in life science research as an organic compound or biological material.
Additional Infomation	P-nitrophenyl butyrate is a butyrate ester resulting from the formal condensation of the hydroxy group of 4-nitrophenol with the carboxy group of butyric acid. It is a butyrate ester and a C-nitro compound. It is functionally related to a 4-nitrophenol.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (478.01 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (11.95 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.95 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.7801 mL	23.9006 mL	47.8011 mL
	5 mM	0.9560 mL	4.7801 mL	9.5602 mL
	10 mM	0.4780 mL	2.3901 mL	4.7801 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.