4-Nitrophenyl β-D-xylopyranoside is a chromogenic β-xylosidase substrate. 4-Nitrophenyl β-D-xylopyranoside may be utilized to detect β-xylosidase activity.

Physicochemical Properties

Molecular Formula	C11H13NO7
Molecular Weight	271.22
Exact Mass	271.069
CAS #	2001-96-9
PubChem CID	91509
Appearance	White to light yellow solid powder
Density	1.597g/cm3
Boiling Point	523.2ºC at 760 mmHg
Melting Point	160 °C
Flash Point	270.2ºC
Vapour Pressure	8.94E-12mmHg at 25°C
Index of Refraction	1.654
LogP	-0.3
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	2
Heavy Atom Count	19
Complexity	313
Defined Atom Stereocenter Count	4
SMILES	C1[C@H]([C@@H]([C@H]([C@@H](O1)OC2=CC=C(C=C2)[N+](=O)[O-])O)O)O
InChi Key	MLJYKRYCCUGBBV-YTWAJWBKSA-N
InChi Code	InChI=1S/C11H13NO7/c13-8-5-18-11(10(15)9(8)14)19-7-3-1-6(2-4-7)12(16)17/h1-4,8-11,13-15H,5H2/t8-,9+,10-,11+/m1/s1
Chemical Name	(2S,3R,4S,5R)-2-(4-nitrophenoxy)oxane-3,4,5-triol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	At high concentrations of 4-Nitrophenyl β-D-xylopyranoside and with xylo-oligosaccharides, β-D-xylan xylohydrolase demonstrates glycosyltransferase activity[1].
References	[1]. The beta-D-xylosidase of Trichoderma reesei is a multifunctional beta-D-xylan xylohydrolase. Biochem J. 1997 Jan 15;321 ( Pt 2)(Pt 2):375-81.
Additional Infomation	4-nitrophenyl beta-D-xyloside is a xyloside that is beta-D-xylopyranose in which the anomeric hydroxy hydrogen is replaced by a 4-nitrophenyl group. It has a role as a chromogenic compound. It is a xyloside and a C-nitro compound. It is functionally related to a 4-nitrophenol.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (368.70 mM) H2O: 50 mg/mL (184.35 mM）

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.22 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.22 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (9.22 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.6870 mL	18.4352 mL	36.8704 mL
	5 mM	0.7374 mL	3.6870 mL	7.3741 mL
	10 mM	0.3687 mL	1.8435 mL	3.6870 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.