4-Methylumbelliferyl-α-D-galactopyranoside (4MU-α-Gal) is a blue fluorescent substrate of α-galactosidase A (GLA). 4-Methylumbelliferyl-α-D-galactopyranoside will form two products, galactose and fluorescent 4MU, after being cleaved by GLA.

Physicochemical Properties

Molecular Formula	C16H18O8
Molecular Weight	338.31
Exact Mass	338.1
CAS #	38597-12-5
PubChem CID	2733788
Appearance	White to off-white solid powder
Density	1.5±0.1 g/cm3
Boiling Point	626.9±55.0 °C at 760 mmHg
Melting Point	171-173ºC
Flash Point	233.9±25.0 °C
Vapour Pressure	0.0±1.9 mmHg at 25°C
Index of Refraction	1.641
LogP	-0.46
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	3
Heavy Atom Count	24
Complexity	506
Defined Atom Stereocenter Count	5
SMILES	CC1=CC(=O)OC2=C1C=CC(=C2)O[C@@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O
InChi Key	YUDPTGPSBJVHCN-CHUNWDLHSA-N
InChi Code	InChI=1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m1/s1
Chemical Name	4-methyl-7-[(2R,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The wavelength of emission for 4-Methylumbelliferyl-α-D-galactopyranoside (4MU-α-Gal) is 440 nm, while the wavelength of excitation is 365 nm [1].
References	[1]. High throughput screening for inhibitors of alpha-galactosidase. Curr Chem Genomics. 2010 Dec 3;4:67-73.
Additional Infomation	4-methylumbelliferyl alpha-D-galactoside is an alpha-D-galactoside having a 4-methylumbelliferyl substituent at the anomeric position It has a role as a chromogenic compound. It is a member of coumarins, an alpha-D-galactoside and a monosaccharide derivative. It is functionally related to a 4-methylumbelliferone.

Solubility Data

Solubility (In Vitro)

DMSO: 33.33 mg/mL (98.52 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9559 mL	14.7793 mL	29.5587 mL
	5 mM	0.5912 mL	2.9559 mL	5.9117 mL
	10 mM	0.2956 mL	1.4779 mL	2.9559 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.