4-Hydroxymethylpyrazole is the primary metabolite of Fomepizole . Fomepizole is a competitive alcohol dehydrogenase inhibitor.

Physicochemical Properties

Molecular Formula	C₄H₆N₂O
Molecular Weight	98.10
Exact Mass	98.048
CAS #	25222-43-9
PubChem CID	3015210
Appearance	White to off-white solid powder
Density	1.3±0.1 g/cm3
Boiling Point	326.8±17.0 °C at 760 mmHg
Flash Point	151.5±20.9 °C
Vapour Pressure	0.0±0.7 mmHg at 25°C
Index of Refraction	1.590
LogP	-0.87
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	1
Heavy Atom Count	7
Complexity	57.7
Defined Atom Stereocenter Count	0
InChi Key	JRMKJOOJKCAEJK-UHFFFAOYSA-N
InChi Code	InChI=1S/C4H6N2O/c7-3-4-1-5-6-2-4/h1-2,7H,3H2,(H,5,6)
Chemical Name	1H-pyrazol-4-ylmethanol
Synonyms	4Hydroxymethylpyrazole; 4 Hydroxymethylpyrazole
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Pyrazoles as Inhibitors of Alcohol Oxidation and as Important Tools in Alcohol Research: An Approach to Therapy Against Methanol Poisoning. Proc Natl Acad Sci U S A . 1979 Jul;76(7):3499-503.
Additional Infomation	4-Hydroxymethylpyrazole is a member of pyrazoles.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~1019.37 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (25.48 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (25.48 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (25.48 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	10.1937 mL	50.9684 mL	101.9368 mL
	5 mM	2.0387 mL	10.1937 mL	20.3874 mL
	10 mM	1.0194 mL	5.0968 mL	10.1937 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.