4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F) is a sensitive and specific fluorescent reagent for the detection of thiols. 4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole has low fluorescence background and good fluorophore stability.

Physicochemical Properties

Molecular Formula	C6H4FN3O3S
Molecular Weight	217.1777
Exact Mass	216.995
CAS #	91366-65-3
PubChem CID	122067
Appearance	Yellow to green solid powder
Density	1.7±0.1 g/cm3
Boiling Point	408.8±55.0 °C at 760 mmHg
Melting Point	145-146ºC
Flash Point	201.0±31.5 °C
Vapour Pressure	0.0±1.0 mmHg at 25°C
Index of Refraction	1.632
LogP	0.11
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	1
Heavy Atom Count	14
Complexity	317
Defined Atom Stereocenter Count	0
InChi Key	XROXHZMRDABMHS-UHFFFAOYSA-N
InChi Code	InChI=1S/C6H4FN3O3S/c7-3-1-2-4(14(8,11)12)6-5(3)9-13-10-6/h1-2H,(H2,8,11,12)
Chemical Name	7-fluoro-2,1,3-benzoxadiazole-4-sulfonamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. New fluorogenic reagent having halogenobenzofurazan structure for thiols: 4-(aminosulfonyl)-7-fluoro-2, 1, 3-benzoxadiazole. Analytical chemistry, 1984, 56(13): 2461-2464.

Solubility Data

Solubility (In Vitro)

DMSO : ~125 mg/mL (~575.56 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (9.58 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (9.58 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.6045 mL	23.0224 mL	46.0448 mL
	5 mM	0.9209 mL	4.6045 mL	9.2090 mL
	10 mM	0.4604 mL	2.3022 mL	4.6045 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.