3-Methyl-L-histidine can be used as a biomarker for meat intake, especially chicken intake, and soy product intake.

Physicochemical Properties

Molecular Formula	C7H11N3O2
Molecular Weight	169.18114
Exact Mass	169.085
CAS #	368-16-1
PubChem CID	64969
Appearance	White to off-white solid powder
Density	1.37g/cm3
Boiling Point	415ºC at 760mmHg
Flash Point	204.8ºC
Index of Refraction	1.613
LogP	0.01
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	3
Heavy Atom Count	12
Complexity	174
Defined Atom Stereocenter Count	1
SMILES	CN1C=NC=C1C[C@@H](C(=O)O)N
InChi Key	JDHILDINMRGULE-LURJTMIESA-N
InChi Code	InChI=1S/C7H11N3O2/c1-10-4-9-3-5(10)2-6(8)7(11)12/h3-4,6H,2,8H2,1H3,(H,11,12)/t6-/m0/s1
Chemical Name	(2S)-2-amino-3-(3-methylimidazol-4-yl)propanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

N(pros)-methyl-L-histidine is a L-histidine derivative that is L-histidine substituted by a methyl group at position 3 on the imidazole ring. It has a role as a human metabolite and a Saccharomyces cerevisiae metabolite. It is a L-histidine derivative and a non-proteinogenic L-alpha-amino acid. It is a tautomer of a N(pros)-methyl-L-histidine zwitterion. 3-Methyl-L-histidine has been reported in Apis cerana, Trypanosoma brucei, and Caenorhabditis elegans with data available. 3-Methylhistidine is a post-translationally modified amino acid found in actin and myosin and a derivative of histidine that has a methyl group bound to the nitrogen at position 3. Urinary levels of 3-methylhistidine are proportional to protein turnover and can be an indicator for myofibrillar protein degradation or a high protein diet.

Solubility Data

Solubility (In Vitro)	H2O : ~100 mg/mL (~591.09 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (295.54 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.9109 mL	29.5543 mL	59.1086 mL
	5 mM	1.1822 mL	5.9109 mL	11.8217 mL
	10 mM	0.5911 mL	2.9554 mL	5.9109 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.