3-Epiursolic Acid is a triterpenoid that can be extracted from Eriobotrya japonica. It is a competitive inhibitor of cathepsin L (cathepsin L). The IC50 and Kis are 6.5 and 19.5 μM respectively. It has no obvious effect on cathepsin B. effect.

Physicochemical Properties

Molecular Formula	C30H48O3
Molecular Weight	456.7003
Exact Mass	456.36
CAS #	989-30-0
PubChem CID	7163177
Appearance	White to off-white solid powder
Density	1.1±0.1 g/cm3
Boiling Point	556.9±50.0 °C at 760 mmHg
Flash Point	304.7±26.6 °C
Vapour Pressure	0.0±3.4 mmHg at 25°C
Index of Refraction	1.555
LogP	9.01
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	1
Heavy Atom Count	33
Complexity	874
Defined Atom Stereocenter Count	10
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Triterpenoids as novel natural inhibitors of human cathepsin L. Chem Biodivers. 2014 Sep;11(9):1354-63.
Additional Infomation	3-Epiursolic acid has been reported in Scutellaria discolor, Salvia officinalis, and other organisms with data available. A pentacyclic triterpene that co-occurs with its isomer OLEANOLIC ACID in several plant species, and occurs in large amounts in FRUITS (such as CRANBERRIES; PEARS; PLUMS; and OLIVES), MEDICINAL HERBS, and other plants.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~109.48 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 1.25 mg/mL (2.74 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.25 mg/mL (2.74 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.25 mg/mL (2.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1896 mL	10.9481 mL	21.8962 mL
	5 mM	0.4379 mL	2.1896 mL	4.3792 mL
	10 mM	0.2190 mL	1.0948 mL	2.1896 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.