3′-Deoxy-3′-fluoroguanosine has inhibitory activity against tick-borne encephalitis virus (TBEV). 3′-Deoxy-3′-fluoroguanosine inhibits viral RNA synthesis by interacting with the NS5B RdRp of HCV, disrupting the further elongation of replicating viral RNA.

Physicochemical Properties

Molecular Formula	C10H12FN5O4
Molecular Weight	285.23
CAS #	123402-21-1
Appearance	Solid powder
SMILES	F[C@]1([H])[C@@]([H])(C([H])([H])O[H])O[C@]([H])([C@]1([H])O[H])N1C([H])=NC2C(N([H])C(N([H])[H])=NC1=2)=O
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Broad-Spectrum Antiviral Activity of 3'-Deoxy-3'-Fluoroadenosine against Emerging Flaviviruses. Antimicrob Agents Chemother. 2021 Jan 20;65(2):e01522-20.

Solubility Data

Solubility (In Vitro)

DMSO : ~20 mg/mL (~70.12 mM; with ultrasonication)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2 mg/mL (7.01 mM)(saturation unknown) in 10% DMSO 40% PEG300 5% Tween-80 45% Saline (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution and add it to 400 μL PEG300, mix well; then add 50 μL Tween-80 to the above system, mix well; then continue to add 450 μL of normal saline to make up to 1 mL. Preparation of normal saline: Dissolve 0.9 g of sodium chloride in ddH₂O and make up to 100 mL to obtain a clear and transparent normal saline solution. Solubility in Formulation 2: ≥ 2 mg/mL (7.01 mM)(saturation unknown) in 10% DMSO 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution and add it to 900 μL of 20% SBE-β-CD saline solution and mix well. 2 g SBE-β-CD (sulfobutyl ether β-cyclodextrin) powder is diluted to 10 mL of saline and completely dissolved until clear and transparent. Solubility in Formulation 3: ≥ 2 mg/mL (7.01 mM)(saturation unknown) in 10% DMSO 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.0 mg/mL clear DMSO stock solution and add it to 900 μL corn oil and mix well.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.5059 mL	17.5297 mL	35.0594 mL
	5 mM	0.7012 mL	3.5059 mL	7.0119 mL
	10 mM	0.3506 mL	1.7530 mL	3.5059 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.