3-Amino-2-oxazolidinone (AOZ) is the metabolite of furazolidinone. 3-Amino-2-oxazolidinone as an indicator for the detection of furazolidinone residues.

Physicochemical Properties

Molecular Formula	C3H6N2O2
Molecular Weight	102.09
Exact Mass	102.042
CAS #	80-65-9
Related CAS #	3-Amino-2-oxazolidinone-d4;1188331-23-8
PubChem CID	65725
Appearance	Colorless to off-white solid powder
Density	1.4±0.1 g/cm3
Boiling Point	167.2±23.0 °C at 760 mmHg
Melting Point	65-67ºC
Flash Point	54.9±22.6 °C
Vapour Pressure	1.7±0.3 mmHg at 25°C
Index of Refraction	1.518
LogP	-1.75
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	0
Heavy Atom Count	7
Complexity	92.9
Defined Atom Stereocenter Count	0
InChi Key	KYCJNIUHWNJNCT-UHFFFAOYSA-N
InChi Code	InChI=1S/C3H6N2O2/c4-5-1-2-7-3(5)6/h1-2,4H2
Chemical Name	3-amino-1,3-oxazolidin-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Development and residue screening of the furazolidone metabolite, 3-amino-2-oxazolidinone (AOZ), in cultured fish by an enzyme-linked immunosorbent assay. J Agric Food Chem. 2009 Jul 8;57(13):5687-92. [2]. Rapid and ultrasensitive detection of 3-amino-2-oxazolidinone in catfish muscle with indirect competitive enzyme-linked immunosorbent and immunochromatographic assays. Food and Agricultural Immunology.

Additional Infomation

3-Amino-2-oxazolidone is an oxazolidinone.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (244.88 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (24.49 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (24.49 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (24.49 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	9.7953 mL	48.9764 mL	97.9528 mL
	5 mM	1.9591 mL	9.7953 mL	19.5906 mL
	10 mM	0.9795 mL	4.8976 mL	9.7953 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.