3'-Deoxyuridine-5'-triphosphate trisodium (3'-dUTP trisodium) is a nucleotide analog that strongly and competitively inhibits DNA-dependent RNA polymerases I and II. Inhibits UTP incorporation into RNA with a Ki of 2.0 μM.

Physicochemical Properties

Molecular Formula	C9H12N2NA3O14P3
Molecular Weight	534.09
Related CAS #	3'-Deoxyuridine-5'-triphosphate;69199-40-2
Appearance	Solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

3'-Deoxyuridine-5'-triphosphate(3'-dUTP) is effectively synthesized, beginning with cordycepin. UTP incorporation into RNA by RNA polymerases is significantly and competitively inhibited by 3'-deoxyuridine-5'-triphosphate. Because it has no influence on poly(rA) synthesis, 3'-Deoxyuridine-5'-triphosphate will be helpful in molecular investigations on the connection of template and substrate in RNA synthesis with chromatin, isolated nuclei, or permeable cells[1].

References

[1]. Inhibitory Effects of 3'deoxycytidine 5'-triphosphate and 3'-deoxyuridine 5'-triphosphate on DNA-dependent RNA Polymerases I and II Purified From Dictyostelium Discoideum Cells. Nucleic Acids Res. 1981 Jul 10;9(13):3129-38.

Solubility Data

Solubility (In Vitro)	H2O :~20 mg/mL (~37.45 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (93.62 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.8723 mL	9.3617 mL	18.7234 mL
	5 mM	0.3745 mL	1.8723 mL	3.7447 mL
	10 mM	0.1872 mL	0.9362 mL	1.8723 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.