3',4'-Dihydroxyacetophenone (3,4-DHAP) can be extracted from Picea Schrenkiana and can inhibit tyrosinase with IC50 of 10 μM. 3',4'-Dihydroxyacetophenone (3,4-DHAP) is a vasoactive substance and antioxidant.

Physicochemical Properties

Molecular Formula	C₈H₈O₃
Molecular Weight	152.15
Exact Mass	152.047
CAS #	1197-09-7
PubChem CID	14530
Appearance	Light brown to brown solid powder
Density	1.3±0.1 g/cm3
Boiling Point	372.4±27.0 °C at 760 mmHg
Melting Point	117 °C
Flash Point	193.2±20.2 °C
Vapour Pressure	0.0±0.9 mmHg at 25°C
Index of Refraction	1.595
LogP	1.28
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	1
Heavy Atom Count	11
Complexity	155
Defined Atom Stereocenter Count	0
InChi Key	UCQUAMAQHHEXGD-UHFFFAOYSA-N
InChi Code	InChI=1S/C8H8O3/c1-5(9)6-2-3-7(10)8(11)4-6/h2-4,10-11H,1H3
Chemical Name	1-(3,4-dihydroxyphenyl)ethanone
Synonyms	3',4'Dihydroxyacetophenone; 3',4' Dihydroxyacetophenone
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Antimelanogenic Activity of 3,4-dihydroxyacetophenone: Inhibition of Tyrosinase and MITF. Biosci Biotechnol Biochem. 2006 Feb;70(2):532-4. [2]. Autotoxicity and Allelopathy of 3,4-dihydroxyacetophenone Isolated From Picea Schrenkiana Needles. Molecules. 2011 Oct 24;16(10):8874-93.
Additional Infomation	3,4-dihydroxyacetophenone is a member of acetophenones. It has a role as a metabolite. 3',4'-Dihydroxyacetophenone has been reported in Picea obovata, Picea glauca, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~328.62 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (16.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.43 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (16.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.5725 mL	32.8623 mL	65.7246 mL
	5 mM	1.3145 mL	6.5725 mL	13.1449 mL
	10 mM	0.6572 mL	3.2862 mL	6.5725 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.