24-Methylene cycloartanyl ferulate is a γ-oryzanol compound. In human breast cancer cells, 24-Methylene cycloartanyl ferulate encourages parvin-beta expression. A potential ATP-competitive Akt1 inhibitor is 24-Methylenecycloartanyl ferulate (EC50 = 33.3μM)[1].

Physicochemical Properties

Molecular Formula	C41H60O4
Molecular Weight	616.913
Exact Mass	616.449
CAS #	469-36-3
Related CAS #	469-36-3
PubChem CID	9920169
Appearance	White to off-white solid powder
Density	1.1±0.1 g/cm3
Boiling Point	668.4±40.0 °C at 760 mmHg
Flash Point	193.5±20.8 °C
Vapour Pressure	0.0±2.1 mmHg at 25°C
Index of Refraction	1.564
LogP	13.2
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	10
Heavy Atom Count	45
Complexity	1170
Defined Atom Stereocenter Count	9
SMILES	C[C@H](CCC(=C)C(C)C)[C@H]1CC[C@@]2([C@@]1(CC[C@]34[C@H]2CC[C@@H]5[C@]3(C4)CC[C@@H](C5(C)C)OC(=O)/C=C/C6=CC(=C(C=C6)O)OC)C)C
InChi Key	JBSUVXVGZSMGDJ-YVMHCORFSA-N
InChi Code	InChI=1S/C41H60O4/c1-26(2)27(3)10-11-28(4)30-18-20-39(8)34-16-15-33-37(5,6)35(19-21-40(33)25-41(34,40)23-22-38(30,39)7)45-36(43)17-13-29-12-14-31(42)32(24-29)44-9/h12-14,17,24,26,28,30,33-35,42H,3,10-11,15-16,18-23,25H2,1-2,4-9H3/b17-13+/t28-,30-,33+,34+,35+,38-,39+,40-,41+/m1/s1
Chemical Name	[(1S,3R,6S,8R,11S,12S,15R,16R)-7,7,12,16-tetramethyl-15-[(2R)-6-methyl-5-methylideneheptan-2-yl]-6-pentacyclo[9.7.0.01,3.03,8.012,16]octadecanyl] (E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate
Synonyms	24-Methylene cycloartanyl ferulate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Akt1 (EC50 = 33.3 μM)
References	[1]. 24-Methylenecycloartanyl ferulate, a major compound of γ-oryzanol, promotes parvin-beta expression through an interaction with peroxisome proliferator-activated receptor-gamma 2 in human breast cancer cells. Biochem Biophys Res Comm
Additional Infomation	Oryzanol C is a triterpenoid.

Solubility Data

Solubility (In Vitro)

DMSO: ≥12.5 mg/mL (~20.3 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (2.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.6210 mL	8.1049 mL	16.2098 mL
	5 mM	0.3242 mL	1.6210 mL	3.2420 mL
	10 mM	0.1621 mL	0.8105 mL	1.6210 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.