Physicochemical Properties
| Molecular Formula | C6H6NA3O9P |
| Molecular Weight | 322.05 |
| Exact Mass | 321.944 |
| CAS # | 66170-10-3 |
| Related CAS # | L-Ascorbic acid 2-phosphate magnesium;113170-55-1;L-Ascorbic acid 2-phosphate;23313-12-4 |
| PubChem CID | 10990876 |
| Appearance | White to off-white solid powder |
| Melting Point | 84 °C |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 9 |
| Rotatable Bond Count | 3 |
| Heavy Atom Count | 19 |
| Complexity | 357 |
| Defined Atom Stereocenter Count | 2 |
| SMILES | C([C@@H]([C@@H]1C(=C(C(=O)O1)OP(=O)([O-])[O-])[O-])O)O.[Na+].[Na+].[Na+] |
| InChi Key | YRWWOAFMPXPHEJ-OFBPEYICSA-K |
| InChi Code | InChI=1S/C6H9O9P.3Na/c7-1-2(8)4-3(9)5(6(10)14-4)15-16(11,12)13;;;/h2,4,7-9H,1H2,(H2,11,12,13);;;/q;3*+1/p-3/t2-,4+;;;/m0.../s1 |
| Chemical Name | sodium (R)-5-((S)-1,2-dihydroxyethyl)-2-oxido-4-oxo-4,5-dihydrofuran-3-yl phosphate |
| Synonyms | 2-Phospho-L-ascorbic acid trisodium salt VOK-70103 VOK70103VOK 70103523114 |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | While addition of L-ascorbic acid (Asc) only marginally enhanced cell growth, trisodium L-ascorbic acid 2-phosphate (0.1-1.5 mM; media changes every 2 to 3 days for 2 to 3 weeks) greatly stimulated cell growth. stimulation of growth. Cell growth is greatly enhanced by the combination of Asc-2P and bFGF; however, additional effects are not produced by the addition of EGF or insulin [1]. Higher doses of AsA2-P lead to enhanced runx2 expression and ALP activity. L-ascorbic acid 2-phosphate (50 µM-250 µM) trisodium is required for the efficient osteogenic differentiation of human adipose stem cells (hASC). The combinations of 150 µM asA2-P and 10 nM dexamethasone (Dex) and 250 µM asA2-P and 5 nM Dex produced the maximum proliferation, ALP activity, and runx2 expression [3]. |
| References |
[1]. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate.Invest Ophthalmol Vis Sci. 2011 Nov 7;52(12):8711-7. [2]. Epidermal growth factor inhibits transcription of type I collagen genes and production of type I collagen in cultured human skin fibroblasts in the presence and absence of L-ascorbic acid 2-phosphate, a long-acting vitamin C derivative.J Biol Chem. 1991 May 25;266(15):9997-10003. [3]. Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro.Stem Cell Res Ther. 2013 Feb 15;4(1):17. |
Solubility Data
| Solubility (In Vitro) |
H2O : ~150 mg/mL (~465.77 mM) DMSO : ~6 mg/mL (~18.63 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: 120 mg/mL (372.61 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1051 mL | 15.5255 mL | 31.0511 mL | |
| 5 mM | 0.6210 mL | 3.1051 mL | 6.2102 mL | |
| 10 mM | 0.3105 mL | 1.5526 mL | 3.1051 mL |