2-O-Methyl PAF C-16 (ET-18-O-OCH3) is a structural analog of the inflammatory mediator platelet-activating factor (PAF) and a cytotoxic ether lipid. 2-O-Methyl PAF C-16 stimulates the release of TNF-α in mouse macrophages.

Physicochemical Properties

Molecular Formula	C25H54NO6P
Molecular Weight	495.67
CAS #	78858-44-3
SMILES	P(=O)([O-])(OC([H])([H])[C@@]([H])(C([H])([H])OC([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H])OC([H])([H])[H])OC([H])([H])C([H])([H])[N+](C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H]
Synonyms	ET-18-O-OCH3
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Tumor necrosis factor release by murine macrophages stimulated by the cytotoxic ether lipid 1-O-hexadecyl-2-O-methyl-SN-glycero-3-phosphorylcholine (ET-18-O-OCH3). Int J Immunopharmacol. 1994 Apr;16(4):335-43.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0175 mL	10.0874 mL	20.1747 mL
	5 mM	0.4035 mL	2.0175 mL	4.0349 mL
	10 mM	0.2017 mL	1.0087 mL	2.0175 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.