2'-Deoxycytidine HCl (NSC-83251), the hydrochloride salt of 2'-Deoxycytidine, is composed of the purine nucleoside guanine linked by its N9 nitrogen to the C1 carbon of deoxyribose. It is similar to guanosine, but with one hydroxyl group removed from the 2' position of the ribose sugar (making it deoxyribose). If a phosphate group is attached at the 5' position, it becomes deoxyguanosine monophosphate.

Physicochemical Properties

Molecular Formula	C9H14CLN3O4
Molecular Weight	263.6782
Exact Mass	263.067
CAS #	3992-42-5
Related CAS #	2'-Deoxycytidine;951-77-9
PubChem CID	107488
Appearance	White to off-white solid powder
Melting Point	168-170°C
Flash Point	254.8ºC
Index of Refraction	57 ° (C=1, H2O)
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	2
Heavy Atom Count	17
Complexity	355
Defined Atom Stereocenter Count	3
SMILES	C1[C@@H]([C@H](O[C@H]1N2C=CC(=NC2=O)N)CO)O.Cl
InChi Key	LTKCXZGFJFAPLY-OERIEOFYSA-N
InChi Code	InChI=1S/C9H13N3O4.ClH/c10-7-1-2-12(9(15)11-7)8-3-5(14)6(4-13)16-8;/h1-2,5-6,8,13-14H,3-4H2,(H2,10,11,15);1H/t5-,6+,8+;/m0./s1
Chemical Name	4-amino-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one;hydrochloride
Synonyms	2'-Deoxycytidine hydrochloride; 2'-Deoxycytidine monohydrochloride; Deoxycytidine hydrochloride; NSC83251; NSC 83251; NSC-83251;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

https://en.wikipedia.org/wiki/Deoxyguanosine

Solubility Data

Solubility (In Vitro)

H2O : ≥ 41 mg/mL (~155.49 mM) DMSO : ~25 mg/mL (~94.81 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (9.48 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (9.48 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: 50 mg/mL (189.62 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.7925 mL	18.9624 mL	37.9248 mL
	5 mM	0.7585 mL	3.7925 mL	7.5850 mL
	10 mM	0.3792 mL	1.8962 mL	3.7925 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.