2'-Deoxy-2'-fluorocytidine is a nucleoside analogue that is a potent inhibitor of Congo hemorrhagic fever virus (CCHFV) replication. 2'-Deoxy-2'-fluorocytidine can synergize with T705 to enhance the antiviral efficacy of both compounds against CCHFV replication.

Physicochemical Properties

Molecular Formula	C9H12FN3O4
Molecular Weight	245.21
Exact Mass	245.081
CAS #	10212-20-1
PubChem CID	101507
Appearance	White to yellow solid powder
Density	1.8±0.1 g/cm3
Boiling Point	500.1±60.0 °C at 760 mmHg
Melting Point	167ºC
Flash Point	256.2±32.9 °C
Vapour Pressure	0.0±2.9 mmHg at 25°C
Index of Refraction	1.697
LogP	-1.26
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	2
Heavy Atom Count	17
Complexity	386
Defined Atom Stereocenter Count	4
SMILES	C1=CN(C(=O)N=C1N)[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)F
InChi Key	NVZFZMCNALTPBY-XVFCMESISA-N
InChi Code	InChI=1S/C9H12FN3O4/c10-6-7(15)4(3-14)17-8(6)13-2-1-5(11)12-9(13)16/h1-2,4,6-8,14-15H,3H2,(H2,11,12,16)/t4-,6-,7-,8-/m1/s1
Chemical Name	4-amino-1-[(2R,3R,4R,5R)-3-fluoro-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The antiviral properties of 2'-Deoxy-2'-fluorocytidine have been demonstrated by 50% effective concentrations (EC50) against CCHFV and CCHFV/ZsG in Huh7 cells, respectively, of 61 nM and 31 nM. Furthermore, in Huh7 cells, it demonstrates a 50% cytotoxicity concentration (CC50) of >50.0 μM[1].
References	[1]. Identification of 2'-deoxy-2'-fluorocytidine as a potent inhibitor of Crimean-Congo hemorrhagic fever virus replication using a recombinant fluorescent reporter virus. Antiviral Res. 2017 Nov;147:91-99.
Additional Infomation	2'-Deoxy-2'-fluorocytidine is a hydroxypyrimidine.

Solubility Data

Solubility (In Vitro)	H2O : 5 mg/mL (20.39 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (407.81 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.0781 mL	20.3907 mL	40.7814 mL
	5 mM	0.8156 mL	4.0781 mL	8.1563 mL
	10 mM	0.4078 mL	2.0391 mL	4.0781 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.