2,4-Dihydroxypyrimidine-5-carboxylic Acid is an endogenously produced metabolite.

Physicochemical Properties

Molecular Formula	C5H4N2O4
Molecular Weight	156.10
Exact Mass	156.017
CAS #	23945-44-0
PubChem CID	90301
Appearance	White to light yellow solid powder
Density	1.8±0.1 g/cm3
Boiling Point	564.9±60.0 °C at 760 mmHg
Melting Point	283 °C (dec.)(lit.)
Flash Point	295.4±32.9 °C
Vapour Pressure	0.0±1.6 mmHg at 25°C
Index of Refraction	1.705
LogP	-1.43
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	11
Complexity	268
Defined Atom Stereocenter Count	0
InChi Key	ZXYAAVBXHKCJJB-UHFFFAOYSA-N
InChi Code	InChI=1S/C5H4N2O4/c8-3-2(4(9)10)1-6-5(11)7-3/h1H,(H,9,10)(H2,6,7,8,11)
Chemical Name	2,4-dioxo-1H-pyrimidine-5-carboxylic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

Uracil-5-carboxylic acid is a pyrimidinemonocarboxylic acid that is uracil carrying a carboxy group at position 5. It is functionally related to a uracil. It is a conjugate acid of a uracil-5-carboxylate. It is a tautomer of a 2,4-dihydroxypyrimidine-5-carboxylic acid. 2,4-Dihydroxypyrimidine-5-carboxylic acid has been reported in Stevia rebaudiana with data available. See also: Uracil 5-carboxylate (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO: 27.5 mg/mL (176.17 mM) H2O: < 0.1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.75 mg/mL (17.62 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.75 mg/mL (17.62 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 27.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.4061 mL	32.0307 mL	64.0615 mL
	5 mM	1.2812 mL	6.4061 mL	12.8123 mL
	10 mM	0.6406 mL	3.2031 mL	6.4061 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.