2'-O-Methyladenosine is a methylated adenine residue that can be detected in the urine of normal humans and adenosine deaminase (ADA). 2'-O-Methyladenosine has unique anti-hypertensive (blood pressure lowering) activity.

Physicochemical Properties

Molecular Formula	C₁₁H₁₅N₅O₄
Molecular Weight	281.27
Exact Mass	281.112
CAS #	2140-79-6
PubChem CID	102213
Appearance	White to off-white solid powder
Density	1.8±0.1 g/cm3
Boiling Point	623.8±65.0 °C at 760 mmHg
Melting Point	200-202ºC
Flash Point	331.0±34.3 °C
Vapour Pressure	0.0±1.9 mmHg at 25°C
Index of Refraction	1.795
LogP	-0.15
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	3
Heavy Atom Count	20
Complexity	349
Defined Atom Stereocenter Count	4
SMILES	CO[C@@H]1[C@@H]([C@H](O[C@H]1N2C=NC3=C(N=CN=C32)N)CO)O
InChi Key	FPUGCISOLXNPPC-IOSLPCCCSA-N
InChi Code	InChI=1S/C11H15N5O4/c1-19-8-7(18)5(2-17)20-11(8)16-4-15-6-9(12)13-3-14-10(6)16/h3-5,7-8,11,17-18H,2H2,1H3,(H2,12,13,14)/t5-,7-,8-,11-/m1/s1
Chemical Name	(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-2-(hydroxymethyl)-4-methoxyoxolan-3-ol
Synonyms	2'OMethyladenosine; 2' O Methyladenosine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vivo	In rats, 2'-O-Methyladenosine (100, 200 mg/kg, ip, single dose) shows distinct hypotensive effects[2].
Animal Protocol	Animal/Disease Models: Male spontaneously hypertensive rats (SHR; 16 weeks old)[2]. Doses: 100 , 200 mg/kg. Route of Administration: IP, single dose. Experimental Results: demonstrated a significant hypotensive effect in decreasing blood pressure by about 40-50%.
References	[1]. Increased excretion of modified adenine nucleosides by children with adenosine deaminase deficiency. Pediatr Res. 1982 May;16(5):362-9. [2]. Naturally occurring 2'-O-methylpurine nucleosides with hypotensive properties. Cell Mol Life Sci. 1998 Feb;54(2):125-8.
Additional Infomation	Cordysinin B is a member of the class of adenosines that is adenosine in which the hydroxy group at position 2' is replaced by a methoxy group. It has been isolated from the mycelia of Cordyceps sinensis. It has a role as a fungal metabolite. It is a member of adenosines and an ether. It is functionally related to an adenosine. 2'-O-Methyladenosine has been reported in Euglena gracilis, Ophiocordyceps sinensis, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ~66.67 mg/mL (~237.03 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 3 mg/mL (10.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 3 mg/mL (10.67 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 3 mg/mL (10.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.5553 mL	17.7765 mL	35.5530 mL
	5 mM	0.7111 mL	3.5553 mL	7.1106 mL
	10 mM	0.3555 mL	1.7777 mL	3.5553 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.