1H-Imidazole-2-carbaldehyde is a biochemical compound that may be utilized as a biomaterial or organic/chemical reagent for biomedical research.

Physicochemical Properties

Molecular Formula	C4H4N2O
Molecular Weight	96.09
Exact Mass	96.032
CAS #	10111-08-7
PubChem CID	24955
Appearance	Light yellow to yellow solid powder
Density	1.3±0.1 g/cm3
Boiling Point	296.5±23.0 °C at 760 mmHg
Melting Point	209 °C (dec.)(lit.)
Flash Point	137.5±29.0 °C
Vapour Pressure	0.0±0.6 mmHg at 25°C
Index of Refraction	1.620
LogP	-0.33
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	1
Heavy Atom Count	7
Complexity	74.1
Defined Atom Stereocenter Count	0
InChi Key	XYHKNCXZYYTLRG-UHFFFAOYSA-N
InChi Code	InChI=1S/C4H4N2O/c7-3-4-5-1-2-6-4/h1-3H,(H,5,6)
Chemical Name	1H-imidazole-2-carbaldehyde
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

A new protein tyrosine phosphatase 1B (PTP1B) inhibitor called imidazole-2-carboxaldehyde has significant uses in preventing type 2 diabetes. The amino acids β-alanine and 2-aminobenzoic acid are used to create a condensation process that yields ligands with tridentate Schiff base carboxylates. Additionally, it has been engaged in the allylation of aldimines guided by imidazoles.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (260.17 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (26.02 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (26.02 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	10.4069 mL	52.0346 mL	104.0691 mL
	5 mM	2.0814 mL	10.4069 mL	20.8138 mL
	10 mM	1.0407 mL	5.2035 mL	10.4069 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.