11-oxo-mogroside V is a natural sweetener with strong anti-oxidant effect. 11-oxo-mogroside V has a significant inhibitory activity against reactive oxygen species, acting on O2-, H2O2 and *OH, with EC50 of 4.79, 16.52 and 146.17 μg/mL respectively.

Physicochemical Properties

Molecular Formula	C60H100O29
Molecular Weight	1285.4186
Exact Mass	1284.635
CAS #	126105-11-1
PubChem CID	14525331
Appearance	White to off-white solid powder
Density	1.5±0.1 g/cm3
Index of Refraction	1.641
LogP	-1.52
Hydrogen Bond Donor Count	18
Hydrogen Bond Acceptor Count	29
Rotatable Bond Count	20
Heavy Atom Count	89
Complexity	2410
Defined Atom Stereocenter Count	0
InChi Key	CGGWHBLPUUKEJC-UHFFFAOYSA-N
InChi Code	InChI=1S/C60H100O29/c1-23(9-13-35(57(4,5)79)88-55-50(89-54-49(78)43(72)38(67)29(20-63)84-54)45(74)40(69)31(86-55)22-81-52-47(76)42(71)37(66)28(19-62)83-52)24-15-16-58(6)32-12-10-25-26(60(32,8)33(64)17-59(24,58)7)11-14-34(56(25,2)3)87-53-48(77)44(73)39(68)30(85-53)21-80-51-46(75)41(70)36(65)27(18-61)82-51/h10,23-24,26-32,34-55,61-63,65-79H,9,11-22H2,1-8H3
Chemical Name	17-[5-[4,5-dihydroxy-3-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxy-6-hydroxy-6-methylheptan-2-yl]-4,4,9,13,14-pentamethyl-3-[3,4,5-trihydroxy-6-[[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxy-1,2,3,7,8,10,12,15,16,17-decahydrocyclopenta[a]phenanthren-11-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	11-oxo-mogroside V demonstrated a greater scavenging action than mogroside V on O2- (concentration that inhibited 50% of chemiluminescence intensity [EC50] = 4.79 μg/mL) and H2O2 (EC50 = 16.52 μg/mL). 11-oxo-mogroside V exhibits a substantial inhibitory impact on *OH-induced DNA damage, with EC50=3.09 μg/mL[1]. 11-oxo-mogroside V is a natural sweetener isolated from monk fruit and has a strong inhibitory action. Induction of Epstein-Barr virus early antigen (EBV-EA) by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) revealed inhibitory effects on preliminary screening assays. 11-oxo-mogroside V revealed substantial inhibitory effects on EBV-EA induction (91.2%, 50.9% and 21.3% inhibition at 1000, 500 and 100 molar ratio/TPA concentrations, respectively) [2].
ln Vivo	Just 26.6% and 53.3% of mice in the groups treated with DMBA, TPA, and 11-oxo-mogroside V developed papillomas even after 10 and 15 weeks of escalation, respectively. Per mouse, only 1.0, 3.3, and 4.7 papillomas developed. There are 15 and 20 week promotions[2].
References	[1]. The antioxidant activities of natural sweeteners, mogrosides, from fruits of Siraitia grosvenori. Int J Food Sci Nutr. 2007 Nov;58(7):548-56. [2]. Anticarcinogenic activity of natural sweeteners, cucurbitane glycosides, from Momordica grosvenori. Cancer Lett. 2003 Jul 30;198(1):37-42.
Additional Infomation	11-oxo-Mogroside V has been reported in Siraitia siamensis with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~77.80 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (1.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (1.94 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (1.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.7780 mL	3.8898 mL	7.7796 mL
	5 mM	0.1556 mL	0.7780 mL	1.5559 mL
	10 mM	0.0778 mL	0.3890 mL	0.7780 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.