1-Thioglycerol, extensively used as a reducing agent in various biochemical and biophysical applications, especially in protein chemistry and molecular biology, where it protects proteins from oxidation and denaturation and can reduce disulfide bonds to thiols base, which can then be modified or analyzed. Additionally, 1-Thioglycerol has been studied for potential medical applications such as as an inhibitor (blocker/antagonist) of cystic fibrosis, where it may help improve lung cell function. It has also been studied for Used in the preparation of metal nanoparticles and as a stabilizer for certain pharmaceutical preparations.

Physicochemical Properties

Molecular Formula	C3H8O2S
Molecular Weight	108.16
Exact Mass	108.024
CAS #	96-27-5
PubChem CID	7291
Appearance	Colorless to light yellow liquid
Density	1.2±0.1 g/cm3
Boiling Point	247.0±0.0 °C at 760 mmHg
Flash Point	126.3±24.6 °C
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.526
LogP	-0.92
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Heavy Atom Count	6
Complexity	32
Defined Atom Stereocenter Count	0
SMILES	OCC(CS)O
InChi Key	PJUIMOJAAPLTRJ-UHFFFAOYSA-N
InChi Code	InChI=1S/C3H8O2S/c4-1-3(5)2-6/h3-6H,1-2H2
Chemical Name	3-sulfanylpropane-1,2-diol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	1-Thioglycerol is a biochemical reagent that can be utilized in research pertaining to life sciences as an organic compound or biological material.
Additional Infomation	Monothioglycerol is a thiol that is glycerol in which one of the primary hydroxy groups is replaced by a thiol group. It has a role as a vulnerary and a reducing agent. It is a thiol and a member of propane-1,2-diols.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (924.56 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (23.11 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	9.2456 mL	46.2278 mL	92.4556 mL
	5 mM	1.8491 mL	9.2456 mL	18.4911 mL
	10 mM	0.9246 mL	4.6228 mL	9.2456 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.