1-Phenyl-2-pyrrolidinone (1-Phenylpyrrolidin-2-one) is a GABA analog that has sedative effects and can reduce exploratory behavior in rats at 50-100 mg/kg (intravenous injection). 1-Phenyl-2-pyrrolidinone has also been shown to suppress emotional responses in dogs and cats. 1-Phenyl-2-pyrrolidinone induces a reduction in the pressor response to emotional stress but does not affect the normal reflex function of baroreceptors.

Physicochemical Properties

Molecular Formula	C10H11NO
Molecular Weight	161.20
Exact Mass	161.084
CAS #	4641-57-0
PubChem CID	78375
Appearance	White to off-white solid powder
Density	1.1±0.1 g/cm3
Boiling Point	355.5±11.0 °C at 760 mmHg
Melting Point	67-69 °C(lit.)
Flash Point	174.7±10.5 °C
Vapour Pressure	0.0±0.8 mmHg at 25°C
Index of Refraction	1.578
LogP	1.39
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	1
Heavy Atom Count	12
Complexity	173
Defined Atom Stereocenter Count	0
InChi Key	JMVIVASFFKKFQK-UHFFFAOYSA-N
InChi Code	InChI=1S/C10H11NO/c12-10-7-4-8-11(10)9-5-2-1-3-6-9/h1-3,5-6H,4,7-8H2
Chemical Name	1-phenylpyrrolidin-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Some psychotropic effects of phenibut and phenylpyrrolidone. Trudy Volgogradskogo Gosudarstvennogo Meditsinskogo Instituta (1979), 31(3), 63-6. [2]. Effect of γ-aminobutyric acid derivatives on baroreceptor reflexes in nonanesthetized animals. Russian. 1980, (VINITI 1311-80), 10 pp.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (620.35 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (15.51 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (15.51 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (15.51 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.2035 mL	31.0174 mL	62.0347 mL
	5 mM	1.2407 mL	6.2035 mL	12.4069 mL
	10 mM	0.6203 mL	3.1017 mL	6.2035 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.