1-Hydroxypyrene is a biomarker of exposure to polycyclic aromatic hydrocarbons (PAHs) and is present in urine samples. 1-Hydroxypyrene is the primary biomarker of exposure to pyrenes.

Physicochemical Properties

Molecular Formula	C₁₆H₁₀O
Molecular Weight	218.26
Exact Mass	218.073
CAS #	5315-79-7
PubChem CID	21387
Appearance	Light yellow to light brown solid powder
Density	1.4±0.1 g/cm3
Boiling Point	437.4±14.0 °C at 760 mmHg
Melting Point	179-182ºC(lit.)
Flash Point	213.0±12.0 °C
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.895
LogP	4.43
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Heavy Atom Count	17
Complexity	300
Defined Atom Stereocenter Count	0
InChi Key	BIJNHUAPTJVVNQ-UHFFFAOYSA-N
InChi Code	InChI=1S/C16H10O/c17-14-9-7-12-5-4-10-2-1-3-11-6-8-13(14)16(12)15(10)11/h1-9,17H
Chemical Name	pyren-1-ol
Synonyms	1Hydroxypyrene; 1 Hydroxypyrene
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Urinary 1-hydroxypyrene Is Associated With Oxidative Stress and Inflammatory Biomarkers in Acute Myocardial Infarction. Int J Environ Res Public Health.
Additional Infomation	1-Hydroxypyrene is a member of pyrenes.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~229.08 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (11.45 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.45 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.5817 mL	22.9085 mL	45.8169 mL
	5 mM	0.9163 mL	4.5817 mL	9.1634 mL
	10 mM	0.4582 mL	2.2908 mL	4.5817 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.