1-Aminohydantoin HCl is the major metabolite of nitrofurantoin in animal tissues and could be utilized as an indicator for the determination of veterinary drug residues in meat, milk, etc. 1-Aminohydantoin HCl is covalently bound to tissue proteins and is released from tissues under slightly acidic conditions. Nitrophenyl analogues derivatized with 2-nitrobenzaldehyde to form AHD can be detected.

Physicochemical Properties

Molecular Formula	C3H6CLN3O2
Molecular Weight	151.55
Exact Mass	151.014
CAS #	2827-56-7
PubChem CID	12472963
Appearance	White to off-white solid powder
Boiling Point	256.8ºC at 760 mmHg
Melting Point	201-205 °C(lit.)
Flash Point	109.1ºC
Vapour Pressure	0.0119mmHg at 25°C
LogP	0.18
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	0
Heavy Atom Count	9
Complexity	144
Defined Atom Stereocenter Count	0
InChi Key	WEOHANUVLKERQI-UHFFFAOYSA-N
InChi Code	InChI=1S/C3H5N3O2.ClH/c4-6-1-2(7)5-3(6)8;/h1,4H2,(H,5,7,8);1H
Chemical Name	1-aminoimidazolidine-2,4-dione;hydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. A fluorescent immunochromatographic strip test using a quantum dot-antibody probe for rapid and quantitative detection of 1-aminohydantoin in edible animal tissues. Anal Bioanal Chem. 2018 Jan;410(2):565-572. [2]. Development of a lateral flow immunoassay (LFA) strip for the rapid detection of 1-aminohydantoin in meat samples. J Food Sci. 2011 Aug;76(6):T138-43.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (164.96 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (16.50 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.50 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (16.50 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.5985 mL	32.9924 mL	65.9848 mL
	5 mM	1.3197 mL	6.5985 mL	13.1970 mL
	10 mM	0.6598 mL	3.2992 mL	6.5985 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.