1,3,6,8-Tetrahydroxynaphthalene (T4HN) is an indispensable precursor of DHN melanin and a unique symmetric polyketide origin compound.

Physicochemical Properties

Molecular Formula	C10H8O4
Molecular Weight	192.17
Exact Mass	192.042
CAS #	18512-30-6
PubChem CID	440202
Appearance	Light brown to brown solid powder
Density	1.638g/cm3
Boiling Point	407.1ºC at 760mmHg
Flash Point	208.9ºC
Vapour Pressure	3.29E-07mmHg at 25°C
Index of Refraction	1.832
LogP	1.662
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	0
Heavy Atom Count	14
Complexity	188
Defined Atom Stereocenter Count	0
SMILES	C1(O)C2C(=CC(O)=CC=2O)C=C(O)C=1
InChi Key	BCMKHWMDTMUUSI-UHFFFAOYSA-N
InChi Code	InChI=1S/C10H8O4/c11-6-1-5-2-7(12)4-9(14)10(5)8(13)3-6/h1-4,11-14H
Chemical Name	naphthalene-1,3,6,8-tetrol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	It is believed that type 1 polyketide synthase (PKS) directly produces 1,3,6,8-Tetrahydroxynaphthalene (T4HN), an early precursor of the pentaketide pathway, from malonyl-CoA and acetyl-CoA [2].
Additional Infomation	Naphthalene-1,3,6,8-tetrol is a naphthalenetetrol that is naphthalene substituted by hydroxy groups at positions 1, 3, 6 and 8. 1,3,6,8-Tetrahydroxynaphthalene has been reported in Glarea lozoyensis, Nodulisporium, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO: 125 mg/mL (650.47 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (10.82 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.2037 mL	26.0186 mL	52.0373 mL
	5 mM	1.0407 mL	5.2037 mL	10.4075 mL
	10 mM	0.5204 mL	2.6019 mL	5.2037 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.