(±)-α-Tocopherol nicotinate, vitamin E-nicotinate, is an orally bioactive fat-soluble antioxidant that can prevent cell membrane lipid peroxidation. (±)-α-Tocopherol nicotinate can be hydrolyzed into α-tocopherol and nicotinic acid in the blood and may be utilized in study/research of related vascular diseases.

Physicochemical Properties

Molecular Formula	C35H53NO3
Molecular Weight	535.8
Exact Mass	535.402
CAS #	51898-34-1
PubChem CID	27990
Appearance	White to off-white solid powder
Density	1.0±0.1 g/cm3
Boiling Point	649.0±55.0 °C at 760 mmHg
Flash Point	346.3±31.5 °C
Vapour Pressure	0.0±1.9 mmHg at 25°C
Index of Refraction	1.514
LogP	13.23
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	15
Heavy Atom Count	39
Complexity	725
Defined Atom Stereocenter Count	3
SMILES	CC1=C(C(=C(C2=C1O[C@](CC2)(C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)C)OC(=O)C3=CN=CC=C3)C
InChi Key	MSCCTZZBYHQMQJ-AZAGJHQNSA-N
InChi Code	InChI=1S/C35H53NO3/c1-24(2)13-9-14-25(3)15-10-16-26(4)17-11-20-35(8)21-19-31-29(7)32(27(5)28(6)33(31)39-35)38-34(37)30-18-12-22-36-23-30/h12,18,22-26H,9-11,13-17,19-21H2,1-8H3/t25-,26-,35-/m1/s1
Chemical Name	[(2R)-2,5,7,8-tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]-3,4-dihydrochromen-6-yl] pyridine-3-carboxylate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	(±)-α-Tocopherol nicotinate reduces lipid peroxidative stress in red blood cell membranes, enhances hemorrheology, and improves red blood cell deformability, all of which can help patients with type 2 diabetes slow down the development of microvascular illness [1]. (±)-alpha-tocopheryl nicotinate, or vitamin E, at 2 μg/mL for a full day. Thymic epithelial cell line IT45-R1 is pretreated, and immature T cells are subsequently cultured to enhance CD4+ in thymocytes. share of CD8-T cells [2].
ln Vivo	(±)-alpha-tocopherol nicotinate (Vit E) (50 mg/kg or 585 mg/kg, for seven weeks) in animal diet when compared to a low dosage treatment of 50 mg/kg, the high dose concentration of 585 mg/kg dramatically increases the proportion of CD4+CD8- T cells and ICAM- in the thymus 1 expression in epithelial cells (TEC) recovered from male Fisher rats [2].
References	[1]. Reducing lipid peroxidation stress of erythrocyte membrane by alpha-tocopherol nicotinate plays an important role in improving blood rheological properties in type 2 diabetic patients with retinopathy. Diabet Med. 1998 May;15(5):380-5. [2]. Vitamin E enhances T cell differentiation through increased epithelial cell function in rat thymus,Nutrition Research,Volume 17, Issue 5,1997,Pages 873-883.
Additional Infomation	Tocopheryl nicotinate is a tocol.

Solubility Data

Solubility (In Vitro)

DMSO : ~10 mg/mL (~18.66 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1 mg/mL (1.87 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1 mg/mL (1.87 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1 mg/mL (1.87 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.8664 mL	9.3318 mL	18.6637 mL
	5 mM	0.3733 mL	1.8664 mL	3.7327 mL
	10 mM	0.1866 mL	0.9332 mL	1.8664 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.