(+)-N,N'-Diallyl-L-tartardiamide is a crosslinking agent used in the preparation of hydrogels. (+)-N,N'-Diallyl-L-tartardiamide is a biomaterial or organic compound that can be used in life science research.

Physicochemical Properties

Molecular Formula	C10H16N2O4
Molecular Weight	228.25
CAS #	58477-85-3
PubChem CID	94074
Appearance	Typically exists as solids at room temperature
Density	1.192 g/cm3
Boiling Point	566.8ºC at 760 mmHg
Melting Point	186-188 °C(lit.)
Flash Point	296.6ºC
Index of Refraction	108 ° (C=2.4, H2O)
LogP	0
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	7
Heavy Atom Count	16
Complexity	250
Defined Atom Stereocenter Count	2
SMILES	O([H])[C@@]([H])(C(N([H])C([H])([H])C([H])=C([H])[H])=O)[C@@]([H])(C(N([H])C([H])([H])C([H])=C([H])[H])=O)O[H]
Synonyms	58477-85-3; N,N'-Diallyltartardiamide; DTXSID60886266; Butanediamide, 2,3-dihydroxy-N1,N4-di-2-propen-1-yl-, (2R,3R)-; Butanediamide, 2,3-dihydroxy-N,N'-di-2-propenyl-, (R-(R*,R*))-; DTXCID401025601; 261-277-3; N,N'-Diallyl-L-tartardiamide;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Biochemical Assay Reagent
References	[1]. Biochemical reagentsM//Methods of Enzymatic Analysis. Academic Press, 1965: 967-1037.
Additional Infomation	Enzymes, coenzymes and metabolites are the reagents characteristic of enzymatic analysis. Some of these materials must be prepared or isolated in the laboratory, but many are available commercially. Information about these materials is less readily available to the analyst than information about inorganic reagents. This situation is made worse by the multiplicity and variety of the definitions of purity. In 1955, the Committee on Biological Chemistry and Chemical Technology, National Academy of Sciences, National Research Council (USA) began to collect standard values for biochemical compounds1). As a result of this work the first volume of “Specifications and Criteria of Biochemical Compounds” was published in June 1960*). This volume does not contain chapters on “Phosphate Esters” and “Enzymes”. This chapter examines the activity and the stability of biochemical reagents. The best criterion for the purity of an enzyme is the specific activity together with details of the activity of contaminating enzymes. The activity of these contaminants is expressed as a percentage of the specific activity of the enzyme described. The activities of the enzymes are given in units and the specific activity in units per mg protein. To conform to the proposed International Unit, a unit of enzyme activity is defined as the amount of enzyme which transforms 1 μmole of substrate in 1 min at 25°C. ADH contains up to 40 SH groups and 5 zinc atoms per molecule and therefore, it is sensitive to oxidizing and chelating agents. The crystalline suspension in 2.0 M (NH4)2SO4 solution containing 3% Na4P2O7 and 1% glycine is stable for several months at 0°C to 4°C when air is excluded, while at room temperature it is only stable for 1 week. Solutions or suspensions can be dialyzed against distilled water at 0°C to 3°C without any significant loss of activity. Triosephosphate isomerase is used as the auxiliary enzyme and glycerol-1-phosphate dehydrogenase as the indicator enzyme.[1]

Solubility Data

Solubility (In Vitro)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.3812 mL	21.9058 mL	43.8116 mL
	5 mM	0.8762 mL	4.3812 mL	8.7623 mL
	10 mM	0.4381 mL	2.1906 mL	4.3812 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.