Armepavine is a bioactive compound from Nelumbo nucifera. It not only has anti~inflammatory effects on human peripheral blood mononuclear cells, but also has immunosuppressive effects on T lymphocytes and mice with lupus nephritis. Armepavine inhibits TNF-α-induced MAPK and NF-κB signaling cascades.

Physicochemical Properties

Molecular Formula	C19H23NO3
Molecular Weight	313.397
Exact Mass	313.168
Elemental Analysis	C, 72.82; H, 7.40; N, 4.47; O, 15.32
CAS #	524-20-9
Related CAS #	524-20-9
PubChem CID	442169
Appearance	White to off-white solid powder
Density	1.151g/cm3
Boiling Point	460.5ºC at 760 mmHg
Melting Point	149-150 °C
Flash Point	232.3ºC
Index of Refraction	1.584
LogP	3.119
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	4
Heavy Atom Count	23
Complexity	370
Defined Atom Stereocenter Count	1
SMILES	CN1CCC2=CC(=C(C=C2C1CC3=CC=C(C=C3)O)OC)OC
InChi Key	ZBKFZIUKXTWQTP-QGZVFWFLSA-N
InChi Code	InChI=1S/C19H23NO3/c1-20-9-8-14-11-18(22-2)19(23-3)12-16(14)17(20)10-13-4-6-15(21)7-5-13/h4-7,11-12,17,21H,8-10H2,1-3H3/t17-/m1/s1
Chemical Name	4-[[(1R)-6,7-dimethoxy-2-methyl-3,4-dihydro-1H-isoquinolin-1-yl]methyl]phenol
Synonyms	(-)-Armepavine; Evoeuropine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	p65
References	[1]. Inhibitory effects of armepavine against hepatic fibrosis in rats. J Biomed Sci. 2009 Sep 2;16:78.
Additional Infomation	Armepavine is a member of isoquinolines. (-)-Armepavine has been reported in Papaver armeniacum, Discaria chacaye, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO: ~100 mg/mL (~319.1 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.98 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.98 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (7.98 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.1908 mL	15.9541 mL	31.9081 mL
	5 mM	0.6382 mL	3.1908 mL	6.3816 mL
	10 mM	0.3191 mL	1.5954 mL	3.1908 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.