(22S,23S)-Homobrassinolide is one of the most active brassinosteroids inducing plant growth in various plant bioassay systems. (22S,23S)-Homobrassinolide displays Akt-dependent anabolic activity in rat skeletal muscle cells. orally bioactive.

Physicochemical Properties

Molecular Formula	C29H50O6
Molecular Weight	494.70
Exact Mass	494.36
CAS #	80483-89-2
PubChem CID	11409314
Appearance	White to off-white solid powder
Density	1.1±0.1 g/cm3
Boiling Point	643.0±55.0 °C at 760 mmHg
Melting Point	193-194ºC
Flash Point	203.2±25.0 °C
Vapour Pressure	0.0±4.3 mmHg at 25°C
Index of Refraction	1.533
LogP	3.65
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	6
Heavy Atom Count	35
Complexity	770
Defined Atom Stereocenter Count	13
SMILES	CC[C@H]([C@@H]([C@H]([C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2COC(=O)[C@@H]4[C@@]3(C[C@H]([C@H](C4)O)O)C)C)O)O)C(C)C
InChi Key	HJIKODJJEORHMZ-DNVPGCTHSA-N
InChi Code	InChI=1S/C29H50O6/c1-7-17(15(2)3)26(33)25(32)16(4)19-8-9-20-18-14-35-27(34)22-12-23(30)24(31)13-29(22,6)21(18)10-11-28(19,20)5/h15-26,30-33H,7-14H2,1-6H3/t16-,17-,18-,19+,20-,21-,22+,23-,24+,25-,26-,28+,29+/m0/s1
Chemical Name	(1S,2R,4R,5S,7S,11S,12S,15R,16S)-15-[(2S,3S,4S,5S)-5-ethyl-3,4-dihydroxy-6-methylheptan-2-yl]-4,5-dihydroxy-2,16-dimethyl-9-oxatetracyclo[9.7.0.02,7.012,16]octadecan-8-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	(22S,23S)-Homobrassinolide increases the absorption of sucrose and alpha-aminoisobutyric acid while mildly stimulating ferricyanide reduction [2].
References	[1]. Akt-dependent anabolic activity of natural and synthetic brassinosteroids in rat skeletal muscle cells. J Med Chem. 2011;54(12):4057-4066. [2]. Effects of (22S,23S)-Homobrassinolide and Related Compounds on Membrane Potential and Transport of Egeria Leaf Cells. Plant Physiol. 1990;93(3):1268-1271.

Solubility Data

Solubility (In Vitro)

DMSO: 33.33 mg/mL (67.37 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (5.05 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0214 mL	10.1071 mL	20.2143 mL
	5 mM	0.4043 mL	2.0214 mL	4.0429 mL
	10 mM	0.2021 mL	1.0107 mL	2.0214 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.