| Description | X-Gluc cyclohexanamine is a dye used to detect β-glucuronidase (GUS) activity and is commonly used as a marker for gene expression, which can be used to detect the expression of target genes. |
| In vitro | 指南(以下是我们推荐的实验方案。该方案仅供参考,请根据您的具体需求进行修改)。1. 将20 mg X-Gluc环己胺溶解于1mL二甲基甲酰胺(DMF)中,制备X-Gluc母液。2. 将制备好的X-Gluc溶液加入到平板培养基中,最终浓度设定为50 μg/mL,无需灭菌。3. 让平板自然风干后用于接种微生物。4. 将平板在35°C下孵育,并在接种后16-24小时观察结果。 |
| molecular weight | 521.79 |
| Molecular formula | C20H26BrClN2O7 |
| CAS | 114162-64-0 |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| References | 1. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404. |