| Description | Tris-NTA is a His-tagged protein ligand, which can be used to bind His-tagged proteins. |
| In vitro | Tris-NTA, which in presence of Ni 2+ ions can interact with a His10-tag fused to the intracellular C terminus of the receptor. Whereas the Tris-NTA group shows an affinity Kd to bind the His10-tag of ~10-30?nM[1]. |
| molecular weight | 1049.051 |
| Molecular formula | C43H68N8O22 |
| CAS | 1146592-99-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. Moritz Pfreundschuh, et al. Identifying and quantifying two ligand-binding sites while imaging native human membrane receptors by AFM. Nat Commun. 2015 Nov 12;6:8857. 2. Luyao Liu, et al. Rapid and regenerable surface plasmon resonance determinations of biomarker concentration and biomolecular interaction based on tris-nitrilotriacetic acid chips. Anal Chim Acta. 2021 Jul 25;1170:338625. |