| Description | TMRM Perchlorate (T668) is a cell-permeant cationic lipophilic red fluorescent dye (λex=530 nm, λem=592 nm). |
| In vitro | TMRM Perchlorate 是一种荧光探针（激发光，530±21 nm；发射光，592±22 nm）。在TMRM Perchlorate存在下，添加谷氨酸后荧光信号轻微下降，这表明线粒体内膜极化增加。在TMRM Perchlorate（2 μM）存在下，与复合物I底物耦联的呼吸或添加复合物Ⅱ底物后的呼吸减少了27%[1]。低浓度TMRM Perchlorate（50到500 nM）处理海马培养细胞1到3小时，能选择性地染色神经元及其下层的胶质细胞中的线粒体。高浓度TMRM Perchlorate（1到25 μM）处理可快速选择性染色线粒体，并在5到10分钟后达到饱和。TMRM Perchlorate低浓度（50到200 nM）不诱导细胞凋亡，而较高浓度（0.5和2.5 μM）则增强细胞凋亡（KD=500 nM）[2]。 |
| Synonyms | 四甲基若丹明甲酯酸铵, T668 |
| molecular weight | 500.93 |
| Molecular formula | C25H25ClN2O7 |
| CAS | 115532-50-8 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 11 mg/mL (21.96 mM), Sonication is recommended. |
| References | 1. Chowdhury SR, et al. Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep. 2015 Dec 8;36(1):e00286. 2. Monteith A, et al. Imaging of mitochondrial and non-mitochondrial responses in cultured rat hippocampal neurons exposed to micromolar concentrations of TMRM. PLoS One. 2013;8(3):e58059. |