| Description | Thioflavin T (Basic Yellow 1) is a widely used dye for visualizing and is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections. |
| In vitro | Thioflavin T (ThT) 是一种苯并噻唑染料。绑定于淀粉样纤维时，Thioflavin T (ThT) 的荧光增强，因而常用于诊断淀粉样纤维，适用于体外和体内实验。Thioflavin T 的荧光激发和发射变化还依赖于胶束的形成。利用原子力显微镜直接观察到直径为3 nm的Thioflavin T胶束，并沿着代表性纤维的长度观察到结合的Thioflavin T胶束。Thioflavin T胶束与淀粉样纤维结合，导致荧光发射增强[1]。在水溶液中，Thioflavin T以常用于通过荧光测定法监测纤维的浓度（约10-20 μM）形成胶束。通过测量不同浓度的Thioflavin T的特定电导率变化，计算出关键胶束浓度为4.0±0.5 μM。Thioflavin T浓度超过关键胶束浓度时，沿淀粉样纤维长度结合的胶束数量增多。在低pH条件下，如原子力显微镜所观察到的，Thioflavin T胶束受到破坏，以及绑定至淀粉样纤维的Thioflavin T的荧光增强在pH降至3以下时减少数倍。 |
| Synonyms | Basic Yellow 1, 硫黄素 T, 硫黄素T |
| molecular weight | 318.86 |
| Molecular formula | C17H19ClN2S |
| CAS | 2390-54-7 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 12.5 mg/mL (39.20 mM), Sonication is recommended. DMSO: 50 mg/mL (156.81 mM) |
| References | 1. Khurana R, et al. Mechanism of thioflavin T binding to amyloid fibrils. J Struct Biol. 2005 Sep;151(3):229-38. |
| Citations | 1. Deng J L, Huang L F, Bian Z Y, et al.A new neuroprotective candidate TJ1 targeting amyloidogenesis in 5xFAD Alzheimer’s disease mice.International Immunopharmacology.2024, 138: 112653. |