| Description | Tempone is an aminooxyalkane that acts as a free radical scavenger and reacts with reactive oxygen species (ROS). Intravenous Tempone reduces mean arterial pressure in spontaneously hypertensive rats. |
| In vivo | Tempone (100 mg/kg i.v. bolus followed by 30 mg/kg i.v. infusion; rats) significantly improved both glomerular and tubular function (serum urea, creatinine, creatinine clearance, and fractional excretion of Na(+)) in a dose-dependent manner and significantly attenuated the reperfusion-injury associated with I/R (urinary N-acetyl-beta-D-glucosaminidase, aspartate aminotransferase, assessment of renal histology). Tempone also markedly reduced the immunohistochemical evidence of the formation of nitrotyrosine and poly(ADP-ribose), indicating a reduction of nitrosative and oxidative stress, respectively. Importantly, in contrast to its in vivo metabolite TEMPOL (which also provided protective effects against renal I/R and oxidative stress of PT cells), Tempone reduced renal dysfunction and injury without causing a significant reduction in blood pressure upon administration. These results suggest, for the first time, that Tempone can reduce the renal dysfunction and injury caused by I/R and the injury caused to PT cells by oxidative stress without producing the adverse cardiovascular effects observed when using other nitroxyl radicals.[2] |
| Synonyms | 4-Oxo-tempo |
| molecular weight | 170.23 |
| Molecular formula | C9H16NO2 |
| CAS | 2896-70-0 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 45 mg/mL (264.35 mM) PBS (pH 7.2): 10 mg/mL DMF: 30 mg/mL Ethanol: 30 mg/mL |
| References | 1. Snipes W, et al. Electron spinal resonance analysis of the nitroxide spin label 2,2,6,6-tetramethylpipidone-N-oxyl (Tempone) in single crystals of the reduced Tempone matrix. Biophys J. 1974;14(1):20-32. 2. Patel NS, et al. TEMPONE reduces renal dysfunction and injury mediated by oxidative stress of the rat kidney. Free Radic Biol Med. 2002;33(11):1575-1589. |