| Description | Sulforhodamine B sodium salt (Acid Red 52) is a fluorescent dye. It can be used from laser-induced fluorescence to the quantification of cellular proteins of cultured cells. |
| In vitro | Sulforhodamine B（SRB）常作为一种不可穿透膜的极性示踪剂使用，或用于通过细胞蛋白质的测定来确定细胞密度（细胞毒性试验）。SRB试验被广泛用于以低成本进行各种筛选试验，以研究基于细胞的细胞毒性。该协议可以分为四个主要步骤：处理的准备、与所选处理的细胞孵育、细胞固定以及SRB染色和吸光度测量。此试验仅限于手动或半自动筛选，能够以高效和敏感的方式用于测试粘附细胞中的化合物。它还适用于评估基因表达调控（基因敲低、基因表达上调）的效果，以及研究miRNA替换对细胞增殖的影响。 |
| Synonyms | Acid Red 52, Kiton Red 620, 磺酰罗丹明B |
| molecular weight | 580.65 |
| Molecular formula | C27H29N2NaO7S2 |
| CAS | 3520-42-1 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: ＜1 mg/mL (insoluble or slightly soluble), Sonication is recommended. H2O: 20 mg/mL (34.44 mM), Sonication is recommended. |
| References | 1. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984. |
| Citations | 1. Malla S, Nyinawabera A, Neupane R, et al.Novel Thienopyrimidine-Hydrazinyl Compounds Induce DRP1-Mediated Non-Apoptotic Cell Death in Triple-Negative Breast Cancer Cells.Cancers.2024, 16(15): 2621. |