| Description | Sulfo-NHS-SS-Biotin sodium (Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate) is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein. |
| In vitro | Sulfo-NHS-SS-biotin 是一种带负电荷的试剂，不能穿透细胞膜。它与一级胺（NH2），如赖氨酸侧链或多肽的氨基末端发生反应[1]。在单层培养的细胞中，使用Sulfo-NHS-SS-biotin（1 mg/ml；15分钟）进行处理，并用冰冷PBS洗涤。生物素化反应通过在PBS中加入100 mM甘氨酸来终止。随后用PBS洗涤后，利用含有蛋白酶抑制剂的RIPA缓冲液制备细胞提取物[2]。 |
| Target activity | Cell-surface protein: |
| Synonyms | Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate, Sulfo-NThiol-SS-biotin, Sulfo-NHS-SS-Biotin (sodium), Sulfo-NHS-SS-Biotin |
| molecular weight | 606.69 |
| Molecular formula | C19H27N4NaO9S4 |
| CAS | 325143-98-4 |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 180 mg/mL (296.7 mM), Sonication is recommended. DMSO: 100 mg/mL (164.8 mM), Sonication is recommended. |
| References | 1. Chia CP,et al. Phagocytosis in Dictyostelium discoideum is inhibited by antibodies directed primarily against common carbohydrate epitopes of a major cell-surface plasma membrane glycoprotein.Exp Cell Res. 1989 Mar;181(1):11-26. 2. Jo M, et al. Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 2010 Nov 1;70(21):8948-58. |