| Description | Sulfo-NHS-Biotin (Biotin-NHS) is a water-soluble biotinylation reagent used in Biotin-Streptavidin binding reactions. Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins in Streptavidin-Biotin assays. It can be used for protein detection or immobilization when used in conjunction with streptavidin and Immobilized Streptavidin. |
| Synonyms | Sulfosuccinimidobiotin, Sulfosuccinimidyl biotin, Biotin-NHS, Sulfo NHS Biotin, N-hydroxysulfosuccinimidyl biotin |
| molecular weight | 443.42 |
| Molecular formula | C14H18N3NaO8S2 |
| CAS | 119616-38-5 |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 55 mg/ml (124.04 mM) H2O: 20 mg/mL, The compound is unstable in solution. Please use soon. |
| References | 1. Pintado CO, Moreno A, Barbancho M, Pérez de la Lastra JM, Llanes D. The beta chain of the GPIIb molecule on ruminant leukocytes and platelets is not labelled by the sulfo-NHS-biotin method. Vox Sang. 1995;69(3):248-9. 2. Gabant G, Augier J, Armengaud J. Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate. J Mass Spectrom. 2008 Mar;43(3):360-70. PubMed PMID: 17968972. |