| Description | Stains-All is a cationic dye of carbocyanine. |
| In vitro | Calsequestrin、160,000-Da 的糖蛋白和170,000-Da 的蛋白质用 Stains-all 染成蓝色。Stains-all 染色(在 615 nm 处测量)与 Coomassie blue 染色(在 575 nm 处测量)的比例分别为:calsequestrin 1.3、calmodulin 2.0、troponin C 1.4 和 S-100 2.2。因此,Stains-all 不仅能将这些 Ca2+ 结合蛋白染成蓝色,而且相比 Coomassie blue,对这些 Ca2+ 结合蛋白的染色更为敏感[1]。 |
| molecular weight | 559.58 |
| Molecular formula | C30H27BrN2S2 |
| CAS | 7423-31-6 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 9.5 mg/mL (16.98 mM), Sonication and heating to 60℃ are recommended. |
| References | 1. Campbell KP, et al. Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye "Stains-all". J Biol Chem. 1983 Sep 25;258(18):11267-73. |