| Description | Sortin1, a chemical genetic-hit compound, specifically induces mislocalization of both soluble and membrane-associated vacuolar markers in plants and yeast.This compound is unstable in powder form and other related salt forms are recommended. |
| In vitro | Sortin1 was found to redirect the vacuolar destination of plant carboxypeptidase Y and other proteins in Arabidopsis suspension cells and cause these proteins to be secreted. In Arabidopsis seedlings, the application of Sortin1 and -2 led to reversible defects in vacuole biogenesis and root development. Sortin1 treatment of whole Arabidopsis seedlings also resulted in carboxypeptidase Y secretion, indicating that the drug has a similar mode of action in cells and intact plants. Structure-activity relationship studies conducted in Arabidopsis revealed the structural requirements for Sortin1 bioactivity and demonstrated that overlapping Sortin1 substructures can be used to discriminate between vacuolar-flavonoid accumulations and vacuolar-biogenesis defects [1][2]. |
| molecular weight | 441.43 |
| Molecular formula | C26H19NO6 |
| CAS | 503837-98-7 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 100 mg/mL (226.54 mM), Sonication is recommended. |
| References | 1. Zouhar J, et al. Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9497-501. 2. Rosado A, et al. Sortin1-hypersensitive mutants link vacuolar-trafficking defects and flavonoid metabolism in Arabidopsis vegetative tissues. Chem Biol. 2011 Feb 25;18(2):187-97. 3. Orr DJ, et al. 1H NMR-based metabolomics methods for chemical genomics experiments. Methods Mol Biol. 2014;1056:225-39. |