| Description | Rhodamine 6G (Basic Red 1) is a rhodamine analog useful in Pgp efflux assays. It can be widely used in characterizing the kinetics of MRP1- mediated efflux as a laser dye and potential mitochondrial probe. |
| In vitro | Rhodamine 6G（亦称Rhodamine 590）广泛应用于激光介质和荧光示踪剂。该荧光染料能够渗透活细胞，进入细胞后，Rhodamine 6G会与线粒体的内膜结合。基于这些观察，提出Rhodamine染料可用于产生具有低背景噪声和高分辨率的线粒体荧光图像。极低浓度的Rhodamine 6G似乎能够选择性地破坏培养中的恶性细胞，而不影响正常细胞群[2]。 |
| In vivo | 与未经处理的对照组相比，接受Rhodamine 6G治疗的黑色素瘤移植小鼠显示出生存时间延长、肿瘤生长抑制、临床参数改善及转移病灶数量减少。通过测定滚动和粘附的白细胞数量以及这些细胞的总通量，可以监测白细胞与内皮细胞之间相互作用的变化[3]。Rhodamine-6G进入循环系统并标记白细胞。每周两次10-6M Rhodamine 6G的治疗方案显示出最显著的效果[2]。 |
| Synonyms | Basic Red 1, 罗丹明6G, 罗丹明 6G |
| molecular weight | 479.01 |
| Molecular formula | C28H31ClN2O3 |
| CAS | 989-38-8 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 83.3 mg/mL (173.97 mM), Sonication is recommended. DMSO: 7.5 mg/mL (15.66 mM), Sonication is recommended. |
| References | 1. Zehentbauer FM, et al. Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects. Spectrochim Acta A Mol Biomol Spectrosc. 2014;121:147-51. 2. Kutushov M, et al. Low concentrations of Rhodamine 6G selectively destroy tumor cells and improve survival of melanoma transplanted mice. Neoplasma. 2013;60(3):262-73. 3. Jain RK, et al. Measuring leukocyte-endothelial interactions in mice. Cold Spring Harb Protoc. 2013 Jun 1;2013(6):561-3. |