| Description | Pyronin Y (Pyronine G) is an intercalating cationic dye that shows specificity towards RNA. |
| In vitro | Pyronin Y与双链核酸（尤其是RNA）形成荧光复合物，便于流式细胞术中对细胞RNA进行半定量分析，以估计福尔马林固定的EL4白血病肿瘤细胞中每个细胞的RNA含量。该技术已被用来对人类网织红细胞中的RNA进行染色，以便后续的流分析和排序[2]。在活细胞中，该染料也积累在线粒体中。当浓度在1.7至3.3μM时，Pyronin Y几乎专门定位在培养细胞的线粒体中，与另一种线粒体探针，rhodamine 123类似。在该浓度下，PY并不具有毒性，但会抑制细胞增长，导致细胞停滞[3]。 |
| Synonyms | C.I. 45005, 派洛宁Y, 派洛宁 Y, Pyronine G |
| molecular weight | 302.8 |
| Molecular formula | C17H19ClN2O |
| CAS | 92-32-0 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 25 mg/mL (82.56 mM), Sonication is recommended. H2O: 4 mg/mL (13.21 mM), Sonication is recommended. |
| References | 1. Pollack A, et al. Flow cytometric analysis of RNA content in different cell populations using pyronin Yand methyl green. Cytometry. 1982 Jul;3(1):28-35. 2. Tanke HJ, et al. Flow cytometry of human reticulocytes based on RNA fluorescence. Cytometry. 1981 Mar;1(5):313-20. 3. Darzynkiewicz Z, et al. Cytostatic and cytotoxic properties of pyronin Y: relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res. 1986 Nov;46(11):5760-6. 4. Li B, et al. Pyronin Y as a fluorescent stain for paraffin sections. Histochem J. 2002 Jun-Jul;34(6-7):299-303. |