| Description | Propidium Iodide (PI) is a red fluorescent dye that can be used for cell staining for fluorescence microscopy, confocal microscopy, flow cytometry, and fluorometer analysis. In aqueous solution, the Ex/Em of Propidium Iodide is 493/636 nm; upon binding to nucleic acids, the Ex/Em of PI-nucleic acid becomes 535/617 nm and the fluorescence signal is enhanced by 20-30 times. |
| In vitro | 方法：Propidium Iodide 吸收和 Flow cytometry 检测细胞死亡：1、将 PI 原液 (0.5 mg/mL in PBS) 置于 4℃ 黑暗处保存。使用前，立即配制 PI-FACS 缓冲液，每 1 mL PBS 加入 20 µL PI 原液。2、收集悬浮细胞：将细胞直接收集在离心管中，500g 离心 5 min 收获所有细胞。3、收集贴壁细胞：去除并保存含有死亡细胞和有丝分裂细胞的培养基。使用标准的组织培养技术分离活细胞，如用胰蛋白酶-EDTA 孵育，并确保收集任何洗液 (如 PBS)。将培养基中的细胞和来自任何洗涤液的细胞加入到分离的细胞中，500g 离心 5 min 收获所有细胞。4、将收获的细胞重新悬浮在 PI-FACS 缓冲液中。将细胞在室温下黑暗中孵育 15 min。5、用流式细胞仪测定细胞死亡率。[1] |
| Synonyms | PI, 碘化丙啶, 碘化丙啶（PI） |
| molecular weight | 668.39 |
| Molecular formula | C27H34I2N4 |
| CAS | 25535-16-4 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 5 mg/mL (7.48 mM), Sonication and heating to 60℃ are recommended. DMSO: 6.68 mg/mL (10 mM), Sonication is recommended. |
| References | 1. Crowley LC, et al. Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry. Cold Spring Harb Protoc. 2016 Jul 1;2016(7). |
| Citations | 1. Huang F, Liang J, Lin Y, et al.Repurposing of Ibrutinib and Quizartinib as potent inhibitors of necroptosis.Communications Biology.2023, 6(1): 972. |