| Description | Ponceau S (Acid Red 112) is an azo protein dye commonly used as a stain in spot blotting assays for protein quantification. |
| Synonyms | Acid Red 112 |
| molecular weight | 760.57 |
| Molecular formula | C22H12N4Na4O13S4 |
| CAS | 6226-79-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 20 mg/mL (26.30 mM), Sonication is recommended. DMSO: 80 mg/mL (105.18 mM), Sonication is recommended. |
| References | 1. Sander H, et al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem. 2019 Jun 15; 575: 44-53. 2. Litovchick. Staining the Blot for Total Protein with Ponceau S. Cold Spring Harb Protoc. 2020 Mar 2; 2020(3): 098459. 3. Romero-Calvo I, et al. Reversible Ponceau staining as a loading control alternative to actin in Western blots. Anal Biochem. 2010 Jun 15;401(2):318-20. |