Polyinosinic-polycytidylic acid Bioactivity,Chemical Properties,Storage & Solubility Information Pathways-PeptideDB

Description	Polyinosinic-polycytidylic acid (Poly(I:C)) is a double-stranded RNA (dsRNA) and a TLR3 agonist. Polyinosinic-polycytidylic acid induces natural immunity in mammals.
In vitro	方法：子宫颈癌细胞 HeLa、SiHa、C33A 和肺癌细胞 A549 用 Polyinosinic-polycytidylic acid (0.1-1 μg/mL) 处理 24 h，使用 PI Staining 检测细胞死亡情况。结果：Polyinosinic-polycytidylic acid 以剂量依赖性模式有效诱导肿瘤细胞死亡。[1]方法：大鼠星形胶质细胞用 Polyinosinic-polycytidylic acid (10-20 μg/mL) 预处理 12 h，随后暴露于氧-葡萄糖剥夺 (OGD) 条件 12 h，使用 microscopy 检测细胞形态。结果：OGD 诱导了显著的细胞损伤，Polyinosinic-polycytidylic acid 预处理的组中，细胞似乎更健康。Polyinosinic-polycytidylic acid 对培养的星形胶质细胞中 OGD 诱导的损伤具有一定程度的保护作用。[2]
In vivo	方法：为研究在急性缺血模型中的神经保护作用，将 Polyinosinic-polycytidylic acid (0.3 mg/kg) 单次肌肉注射给 Kun-Ming strain 小鼠，2 h 后构建动脉闭塞 (MCAO) 模型。结果：给予 Polyinosinic-polycytidylic acid 显著减轻了缺血性纹状体和皮层的神经功能缺损，减少了梗死体积，并抑制了 TNFα 和 IL-6 水平的升高。[2]方法：为检测体内抗肿瘤活性，将 Polyinosinic-polycytidylic acid (1-100 μg/mouse) 腹腔注射给小鼠皮肤黑色素瘤 B16-F10 诱导的转移性肿瘤 C57BL/6J 小鼠模型。结果：单次给药 Polyinosinic-polycytidylic acid 后，肺肿瘤生长停止。 Polyinosinic-polycytidylic acid 在已建立的肺转移小鼠模型中具有潜在的抗肿瘤活性。[3]
Synonyms	Poly(I:C), 聚胞苷酸
molecular weight	N/A
Molecular formula	(C10H13N4O8P)x.(C9H14N3O8P)x
CAS	24939-03-5
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year
Solubility	H2O: ≥21.5mg/mL DMSO: < 1 mg/mL (insoluble or slightly soluble)
References	1. Meng X, et al. poly(I:C) synergizes with proteasome inhibitors to induce apoptosis in cervical cancer cells. Transl Oncol. 2022 Apr;18:101362. 2. Pan LN, et al. Toll-like receptor 3 agonist Poly I:C protects against simulated cerebral ischemia in vitro and in vivo. Acta Pharmacol Sin. 2012 Oct;33(10):1246-53. 3. Forte G, et al. Polyinosinic-polycytidylic acid limits tumor outgrowth in a mouse model of metastatic lung cancer. J Immunol. 2012 Jun 1;188(11):5357-64.
Citations	1. Li Y, Ma X, Yue Y, et al. Rapid surface display of mRNA antigens by bacteria‐derived outer membrane vesicles for a personalized tumor vaccine. Advanced Materials. 2022: 2109984. 2. Li Y, Ma X, Yue Y, et al. Rapid Surface Display of mRNA Antigens by Bacteria-Derived Outer Membrane Vesicles for a Personalized Tumor Vaccine. Advanced Materials. 2022: 2109984 3. Ma N, Chen Z, Liu G, et al.Normalizing the Immune Macroenvironment via Debulking Surgery to Strengthen Tumor Nanovaccine Efficacy and Eliminate Metastasis.ACS Nano.2022 4. Yang Q, Liu T, Zhou Z, et al.A nanoformulation for immunosuppression reversal and broad-spectrum self-amplifying antitumor ferroptosis-immunotherapy.Biomaterials.2022: 121936.

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Polyinosinic-polycytidylic acid

CAS No.: 24939-03-5