| Description | PIPES (1,4-Piperazinediethanesulfonic acid) serves as a crucial component of the buffering agent in biochemical applications, known as PIPES buffer. |
| In vitro | 为配制pH PIPES缓冲液，将173克1,4-哌嗪二乙磺酸溶解于1升去离子水中。通过加入氢氧化钠颗粒，调节PIPES缓冲液的pH值至6.8。PIPES缓冲液可用于防止戊二醛固定引起的脂质流失和伪影[1]。 |
| Synonyms | 1,4-Piperazinediethanesulfonic acid, 1,4-piperazine bis(ethanesulfonic acid), 1,4-Piperazinebis(ethanesulfonic acid), PIPES |
| molecular weight | 302.37 |
| Molecular formula | C8H18N2O6S2 |
| CAS | 5625-37-6 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: < 1 mg/mL (insoluble） H2O: 5 mg/mL (16.5 mM), when pH is adjusted to 7 with NaOH. Sonication is recommended. |
| References | 1. Jason Moggridge, et al. Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics. Technol Cancer Res Treat. 2017 Dec;16(6):997-1005. |