| Description | PFM01 is an inhibitor of MRE11 endonuclease. PFM01 can regulate double-strand break repair (DSBR) by nonhomologous end-joining (NHEJ) versus homologous recombination (HR). |
| In vitro | PFM01 (100 μM) treatment allows normal DSB repair in HR-defective ATMi-treated or HSC62 (BRCA2-defective) cells[1].RAD51 foci did not form following exo- or endo-inhibitor treatment (mirin or PFM01) in 1BR3 (WT) and HSC62 (BRCA2-defective) cells[1].PFM01 (100 μM) enhances non-homologous end-joining (NHEJ) in H1299 dA3 cells and reduces homologous recombination (HR) in U2OS DR-GFP cells[1].PFM01 substantially relieves the double-strand break (DSB) repair defect confers by mirin or PFM39 in irradiated G2 cells[1]. |
| molecular weight | 293.4 |
| Molecular formula | C14H15NO2S2 |
| CAS | 1558598-41-6 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 4 mg/mL (13.63 mM) |
| References | 1. Shibata A, et, al. DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities. Mol Cell. 2014 Jan 9; 53(1): 7-18. 2. Völkening L, et, al. RAD50 regulates mitotic progression independent of DNA repair functions. FASEB J. 2020 Feb; 34(2): 2812-2820. |